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The insoluble portion was subsequently removed by centrifugation and the supernatant was incubated with Ni-NTA resin (GE Healthcare) for 2 h batch binding at 4 C
[3] That reversibility is a crucial, often-omitted caveat: tesamorelins effect is a maintained pharmacological state, not a one-time fix. We discuss the visceral-fat data and its limits further in our reference on tesamorelins visceral-fat effects and safety profile
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