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Maintenance of cells and animals Ea.hy926 (E9) cells: E9 cells were purchased from National Centre for Cell Science (NCCS), Pune and maintained in Dulbeccos Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Himedia, A002) (10,000 units of penicillin and 10 mg of streptomycin in 0.9% NaCl) in a humidified atmosphere with 5% CO 2 at 37 C

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Research has documented effects on approximately 4,000 genes, with 59% being upregulated (turned up) and 41% being downregulated (turned down)