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The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)

The VEGFR2-Akt-eNOS angiogenic cascade increases blood vessel formation by 129-152% in preclinical studies

[1] It is investigated for its potential role in nitric oxide pathway modulation, growth factor signaling, and extracellular matrix remodeling