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Nitrated HGF ( bar 3 , peroxynitrite-treated at 1:2000) lost its activation activity as demonstrated by a drop in the activation index down to the baseline level observed in the negative-control culture ( bar 1 ) 32 and in GSSSG/LASSS-cultures ( bars 6 and 10 , missing HGF)

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With these results in hand we became convinced that a clinically useful small molecule analog could be designed that possessed the important characteristics of metabolic stability, extended half-live, BBB penetrability and the capability to enhance cognitive functioning