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Samples were incubated at room temperature for 1 h, vortexing intermittently, and then quenched by the addition of hydroxylamine (5 L of 5% w/v in HPLC water per sample) and incubated for 15 min at room temperature
These results suggested that keloid fibroblasts underwent pro-senescence regulation earlier compared with normal skin fibroblasts via p53-pS15 under serum limitation stimulus, and p53-pS15 and FOXO4 translocated from the nucleus to the cytoplasm during FOXO4-DRI treatment
Int J Biochem Cell Biol (2007) 39:4484
However, keeping an elevated intracellular ROS level within AML cells without altering their viability and proliferation potential requires the establishment of a set of detoxification mechanisms to support their continued growth [33]
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