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The severity and duration of your injury influences appropriate protocol length
This is the point where the 28-day clock officially starts ticking
Twice daily application is well-tolerated
That rules out, straightforwardly, radioligand or fluorescent binding assays, competition binding against a known ligand, recombinant receptor-expressing lines, functional readouts tied to a defined coupling pathway, and antagonist controls to demonstrate that an effect is receptor-mediated
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