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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)
The choice between injections and pills depends on individual needs, including deficiency severity and absorption capacity
Proteases in the peritoneal fluid appear to degrade GLP-1 faster than normal, creating a pro-inflammatory environment where the natural braking mechanisms on inflammation are weakened
Activation of NF-B, for instance, promotes the transcription of genes encoding a wide array of pro-inflammatory mediators, including cytokines (e.g., TNF-, IL-1, IL-6, IL-18) and chemokines (e.g., MCP-1, IL-8) (Liu et al., 2020)
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