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Protease activity The quantitative extracellular protease activity in the solution was investigated in Renuspore by employing a commercial EnzChek Protease Assay Kit (Molecular Probes, Eugene, OR, USA), which contains a casein derivative labeled with green-fluorescent BODIPY FL dye

Sample preparation for metabolite analysis The internal standard 2,3,3,3-d 4 -alanine (0.3 mol/sample) was added to each sample before extraction

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3.5 GHK-Cu regulated the SIRT1/STAT3 pathway SIRT1, which is closely associated with cellular metabolism and stress responses, also interacts with the STAT3 pathway (Wu Q