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Fluorescence signals were visualized using a fluorescence microscope (Nikon Ts2R) and quantified by flow cytometry (NovoCyte 3000, Agilent, USA)
Researchers altered the amino acid sequence and attached a fatty acid chain so the molecule survives longer in circulation
Layered on top of glucagon suppression and gastric slowing is a third effect: a centrally mediated reduction in food intake and meal size, mediated through the same area postrema circuitry
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