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The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat
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However, in a high-sugar microenvironment (such as a diabetes-related tumor), TAN Ltf secretion is inhibited by the ROS-JNK pathway, and its specific regulatory network still needs to be further analyzed (219)
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