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For DIA LCMS analysis, 250 ng of peptides from each sample were injected into an HPLC (nanoElute, Bruker Daltonics) and loaded onto a trap column with a constant flow of 4 L/min (Acclaim PepMap100 C18 column, 0.3 mm id 5 mm, Dionex Corporation) then eluted onto an analytical C18 Column (1.9 m beads size, 75 m 25 cm, PepSep) heated at 50 C

In addition, alcohol is metabolized by alcohol dehydrogenase and cytochrome P450-2E1 (ADH and CYP2E1) enzymes found in the brain endothelial cells leading to the production of ROS, which is responsible for oxidative stress and production of neurodegenerative agents (2)

The various treatments for MASHlifestyle modifications, glucagon-like peptide (GLP) 1 receptor agonists, GLP-1 and glucose-dependent insulinotropic polypeptide (GIP) dual agonists, resmetirom, metabolic-bariatric surgery, and metabolic-bariatric endoscopic proceduresreduce steatosis by distinct but converging pathways (Figure 1)