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For in vivo studies, the number of animals used must be kept to an absolute minimum [472]
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Interestingly, considering that CCS52B expression is restricted to early phases of the cell cycle (G2/M and M-phase) unlike the A-types (late M-phase to G2-phase) effect of MTX on CCS52B expression goes in accordance with mediation of mitotic cell cycle arrest at S phase
This varies by peptide and goal
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