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Then, the MB was added at 5-serial dilution concentrations (1.25 to 20 M) and incubated for 24, 48, or 72 h (37 C and 5% CO 2 )
Therefore, diminution of R 2 L caused by exhaustion of R through conversion to RL at an excess amount of ligand (that is, dimeric macrocycles in our case) could be a reason for the bell-shaped responses
Understanding the unique mechanisms and purity of these compounds is paramount for achieving reliable, replicable research outcomes
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