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The harmonic restraints were released in five steps, transitioning the system from an NVT to an NPT ensemble over 100 ps
OCR was recorded three times under basal conditions and three times after the addition of Mito Stress compounds (Mito Stress Test Kit, Agilent, Santa Clara, California, USA): oligomycin (oligo, 1 M, adenosine triphosphate (ATP)-synthase inhibitor), fluorocarbonyl cyanide phenylhydrazone (FCCP, 0.5 M, decoupler), and rotenone/antimycin A (rot/AA, 0.5 M, complex 3/1 inhibitor), as shown in Figure 1
According to a clinical trial published in the New England Journal of Medicine , Tesamorelin significantly reduced VAT and improved lipid profiles in patients with HIV-associated lipodystrophy (Falutz et al., 2010)
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