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Given that both diet composition and adiposity can each substantially impact the gut microflora in obese mice 24 , we conducted a secondary endpoint study using animals given the same series of diets to test the hypothesis that NNMTi treatment combined with LD, but not LD alone, shifts the cecal microbiome profile of obese animals to be similar to age-matched LD controls that were never given a high-fat diet

Pre-dosing with a small protein-rich snack (15-20g protein, minimal fat) 30 minutes before injection can blunt this response without negating the meal-timing strategy

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