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For quantification of sulfur-containing metabolites in cultured cancer cells and mouse serum, 10 l samples were extracted with 90 l of ice-cold extraction solvent (80% methanol and 20% H 2 O) containing isotope-labelled internal standards (36 M 13 C 2 15 N-GSH, 0.92 M 13 C 4 15 N 2 -GSSG and 10 M D 4 -cystine)
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These results suggest that SCFAs and their producing bacteria in the gut may be risk factors for PCa in humans and mice
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