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In brief, 6,250 cells were seeded into 96-well white cell culture-treated plates (Falcon, 353296), treated for 24 h as indicated and labelled following the manufacturers instructions
Importantly, animals were singly housed to eliminate the exchange and cross-contamination of intestinal microbiomes, a factor that potentially biases studies with group-housed animals by minimizing microbiome variations for co-housed animals within a cohort
Any form of alcoholbeer, wine, liquor, etc.should be minimized as well
Your safety and well-being are important, hence the necessity to recognize symptoms of a vaccine injection site reaction and understand when to seek medical help
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