However, doses of 20-40 mg/kg should be limited to a maximum of 6 months
Pre-treatment of HGF with trisulfides and nitration Recombinant mouse HGF (carrier protein-free) was pre-treated with glutathione trisulfide (GSSSG) and lipoic acid trisulfide (LASSS) at mole ratios (HGF : trisulfide) of 1:0 (with solvent alone), 1:400, 1:1000, and 1:4000 at pH 7.4, 25C just prior to the peroxynitrite protocol (optimized at a molar ratio 1:2000 relative to HGF, pH 7.4, 37, for 30 min), which was previously demonstrated to induce maximal nitration of Y198 and Y250 located in the -chain under physiological conditions as described previously 32
Jiang, J., Chan, A., Ali, S., Saha, A., Haushalter, K
GLUTATHIONE BIOSYNTHESIS IN PLANTS The chloroplast, mitochondria, plastid, and cytosol are the key locations for GSH synthesis [14]