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Free fatty acids from either adipose tissue (fat), or from the conversion of excess carbohydrate intake, accumulate in the liver cell resulting in oxidative stress, inflammation, and liver injury
After protein quantification, samples containing equal protein amounts were separated via SDS-PAGE and subsequently transferred to PVDF membranes, following the protocols of Laemmli (1970) and Towbin et al
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Combining peptides that work through different mechanisms can produce additive or synergistic effects
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