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For initializing curve fitting, the background value used was the minimum fluorescence intensity observed at 0 antigen concentration and the EC 50 used was the median antigen concentration tested

Infarct size measurement In mice, at the end of reperfusion period, the LAD was re-ligated, and 5% Evans Blue dye solution (100 l) was infused via jugular vein to determine area at risk

The idea was further validated by inhibiting glutathione synthesis by BSO where Sp administration caused GSH to increase by ~1.5 fold with concomitant reduction in GSSG (Fig

BPC-157: Reinforcing gut integrity naturally BPC-157 takes a different approach