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Reverse transcription of total mRNA were performed at 1 g of total mRNA in 25 l final volume using random primers and avian myeloblastosis virus reverse transcriptase

2.4 Transporter inhibition study using HEK293 cells The procedures for cell culture, dosing solution preparation, cell lysate preparation, and sampling were consistent with those in the substrate study described above, except for the pre-incubation and incubation steps

The spontaneous proliferation induced by HTLV-1 infection depends on intracellular GSH levels

Additionally, our findings showed that they might have been the consequence of gene loss or gain events that happened throughout the evolutionary process