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Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min
Results may vary depending on consistency, lifestyle, dosage, and individual body response
Expression of LaccID and engineered receptors was verified at 96 h after transduction by flow cytometry (day 5)
Mitochondrial activation and reactive oxygen-species overproduction during sperm capacitation are independent of glucose stimuli
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