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RNA extraction and qRTPCR analysis Cells were grown to 80% confluency in 75 cm 2 flasks, harvested by trypsinisation, counted using a haemocytometer, and 1 10 7 cells used for RNA extraction using a Qiagen RNeasy mini kit (Qiagen), following the manufacturers protocol for mammalian cells, with additional on column DNAse digestion (RNAse free DNAse kit, Qiagen)
The primary role of glutathione is to protect your mitochondria
Dani spends her spare time volunteering at an equine rescue or riding rescue horses
In combination with other agents, it can cause gene expression changes that single agents do not
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