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Fixed cells were permeabilized and blocked for 1 h in goat serum dilution buffer (GSDB: 25% goat serum, 0.3% Triton X-100 in PBS)
Vitamin B12 (cobalamin) plays a key role in: Making healthy red blood cells (helping prevent certain types of anaemia) Supporting nerve function Helping normal energy metabolism Supporting cognitive function and mood (when deficiency is present) Because the body doesnt produce B12, we rely on dietary sources (primarily animal products and fortified foods) and on absorption through the gut
Validation of biomarkers of aging
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