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The same needle specifications apply: 30-31 gauge, 4-6mm length, inserted at 90 degrees with a gentle skin pinch
After blocking with 5% BSA, the sections were incubated with 1:200 anti-GLP-1 (ab23468, Abcam), followed by detection with secondary antibodies Alexa Fluor 488 conjugated goat antibody to mouse IgG (A-11029, ThermoFisher
Frontiers in Pharmacology , 12 , 697442
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