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Luciferase activity of each well was measured using the Steady-Glo Luciferase Assay System (Promega) with a Glo-Max Navigator Microplate Luminometer (Promega), according to the manufacturers instruction
Do not leave GHK-Cu at room temperature for extended periods, even before opening
The pore size of cell culture inserts is an important parameter and we compared cell culture inserts with 3 m and 0.4 m pore sizes (Corning Costar
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