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cAMP EC50 0.022 nM per Coskun et al., 2018) similar potency to native GIP Glucagon receptor: minimal binding in reported assays A notable mechanistic nuance: tirzepatide behaves as a biased GIPR agonist in some cellular assays it activates certain downstream pathways (cAMP) more than others (-arrestin recruitment), which may explain why native GIP does not reproduce tirzepatide's in vitro profile (Coskun et al., 2018)
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