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Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)
User 12 used 500mcg daily and was specifically trying to determine whether splitting that dose or taking it all at once produced better results
Since BPC-157 has not been thoroughly tested in humans, its safety profile remains unknown
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