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We determined the duration of the washout period based on the estimated complete turnover time of GSH synthesis in the brain ( Randomization The study randomization and intervention timeline are illustrated in Figure 2
AST (3,3-dihydroxy-,-carotene-4,4-dione) is a xanthophyll carotenoid composed of two terminal -ionone rings linked by a conjugated polyene chain, with a molecular formula of C40H52O4 and 13 conjugated double bonds that contribute to its distinctive red-orange color and antioxidant properties
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Followed by SDS-PAGE electrophoresis, the transfer of proteins was conducted onto PVDF (Millipore) membranes (Solebio, Beijing, China) and immunocontainment experiments were performed after the membrane transfer was completed, the primary antibody was added and incubated at 4 C overnight with slow shaking
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