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To monitor the oxidation of roGFP2 WT by the intramolecular disulfide of PfGrx C88S , 1 M reduced roGFP2 WT was mixed with 2 M diamide-oxidized PfGrx C88S at room temperature
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For example, variant X(GD_L)c.3 (later renamed X(GD_L)f) had a catalytic efficiency of 0.004 M 1 s 1 , whereas the parent X(GD_L)c that lacked reversion and engineering showed no detectable activity on the FRET substrate (Fig
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