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On the barometric method for measurements of ventilation, and its use in small animals

Slices were stained with DAPI (F6057, Sigma, USA) and immunofluorescence images observed under a confocal microscope (Leica, DM6000, Wetzlar, Germany)

1) are dysregulated in response to the GDH2 gene deletion, or these pathways are unable to generate sufficient glutamate inside the cells and the large pool of free NADP + required to overcome the thermodynamic barrier inherent in using the still present Gdh3, which is usually an anabolic enzyme with a higher affinity for -ketoglutarate than for glutamate