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SDSPAGE TTR-V30M samples were combined with a non-reducing 4SDS sample buffer (pH 6.8

To diagnose a Vitamin B12 deficiency, your doctor may run blood tests to see whether you have low haemoglobin or Vitamin B12 levels

Tryptic peptides were automatically loaded on a C18 trap column (300 m inner diameter (ID) x 5 mm, Acclaim PepMap100 C18, 5 m, 100 , LC Packings) at 30 L/min flow rate prior to C18 reversed phase chromatography on the analytical column (nanoEase MZ HSS T3 Column, 100 , 1.8 m, 75 m 250 mm, Waters) at 250 nl/min flow rate in a 95 min nonlinear acetonitrile gradient from 3 to 40% in 0.1% formic acid

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