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Details of saliva collection are described in our paper ( 2.4 Markers of the kynurenine and serotonin pathways, neopterin, biopterin, and creatinine in urine From an ethical standpoint, we opted for non-invasively collected urine (in contrast to invasive blood collection) to determine TRP degradation metabolites, considering the observed concordance between peripheral levels and levels in the central nervous system ( Levels of urine metabolites (creatinine, TRP, KYN, 5-HTP, SER, 5-HIAA, NEO, BIO) were determined using reversed-phase high-performance liquid chromatography (RP-HPLC system, Prominence 20A, Shimadzu Corporation, Japan) equipped with UV-VIS absorption (module SPD-20A) and fluorescence (module RF-10AXL) detectors

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Certain sequences are more susceptible than others, particularly those adjacent to aspartate residues