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Since no commercial cystine-free IMDM is available, we customized IMDM based on a DMEM medium (#D9800-28, USBiological, Salem, MA) without amino acids, glucose, sodium bicarbonate, and sodium pyruvate
Continuous use, especially beyond 6-8 weeks, may lead to diminished effects due to potential receptor desensitization
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The innate immune system responds immediately when it detects danger
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