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The full integration of glutathione biology with the broader concept of cellular redox biology came in 1985, when the German biochemist Helmut Sies, working at the University of Dsseldorf, formulated the conceptual framework of cellular oxidant-and-antioxidant balance that integrated decades of accumulated observation into a coherent picture

Low-yield RNA extraction (for example, following ribosomal pulldown) was carried out using the Absolutely RNA Nanoprep kit (Agilent) as per manufacturers instructions

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