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Its activity profile is best understood as systems-level polypharmacology rather than single-receptor agonism: published research has documented effects on nitric oxide system signalling, growth-factor receptor expression (including VEGF receptor 2 and growth-hormone receptor), dopamine and serotonin pathway markers, and angiogenic factor regulation

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To ensure cell lysis and alkylation of thiol and persulfide species, 200 l of a 5 mM MBB (monobromobimane) solution in 50% methanol was added to the tubules