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Under normal conditions, when you are in a caloric deficit and muscle protein begins breaking down, the amino acid alanine is released from muscle tissue and converted to glucose in the liver through gluconeogenesis
Nutrient intake was measured using a 72 hour food recall, and zinc levels were analysed
As a dietitian, I usually recommend aiming for about 0.7 grams of protein per pound of body weight per day as a starting point (thats around 105 grams daily if you weigh 150 pounds)
Warnings: Do not use if safety seal is broken
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