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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]
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Catechins, derived from green tea, are also eliminated through urine and contribute to bladder protection through antioxidative and anti-inflammatory pathways
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