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HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)
Like NAC, silymarin can protect against acetaminophen toxicity
Sterile needles prevent contamination during vial access procedures
Special Populations Older adults: Prioritize safety, simplicity, and hypoglycaemia avoidance
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