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For immunohistochemical staining, we incubated the sections with primary antibodies overnight at 4 C and used a horseradish peroxidase-streptavidin detection system (Dako) to detect immunoactivity followed by counter-staining with hematoxylin (Sigma-Aldrich)
Hypoxia increases the deoxycytidine triphosphate (dCTP) level through the pentose phosphate pathway (PPP) via glucose metabolism and results in resistance to gemcitabine, a dCTP analog 87
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Theriogenology 217, 8391
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