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Cells were starved for glucose or treated with 25 mM glucose, followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO 2 at 37 C using a STELLARIS 8 FALCON (Leica) system equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica)

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