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Because inhomogeneous cellular distribution of reacted and unreacted probes may bias the ratiometric calculations, especially at the subcellular resolution, we opted for RT instead of RT-NH 2 to achieve high-quality quantitative results in living cells
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Biochimica et Biophysica Acta (BBA) 1842:12401247 Wong HS, Dighe PA, Mezera V, Monternier PA, Brand MD (2017) Production of superoxide and hydrogen peroxide from specific mitochondrial sites under different bioenergetic conditions
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