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Materials and methods Materials Pregnenolone, adenosine 5-triphosphate (ATP), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Trizma base, and dithiothreitol (DTT) were from Sigma-Aldrich (St
They stimulate glucose-dependent insulin secretion from pancreatic beta cells, suppress inappropriate glucagon release from alpha cells, slow gastric emptying, and promote satiety through central nervous system effects
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