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Recombinant Sec TGR Expression and Purification A bacterial-type SECIS element was fused to the TGR open reading frame [14] using PCR with the following oligonucleotides primers: forward 5-catATGCCTCCAGCTGATGGAAC-3 and reverse 5-TCGCCAACGACTCCAATTATTAGCCAACGTCCAGACGTGGTTAGCAATTGGATACGCGGGcagctg-3
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These phosphorylated Smad2 and Smad3 then interact with their co-chaperone Smad4 to form a complex that reaches the nucleus and stimulates the transcription of pro-fibrotic genes[48]
At the end point, 2.5 M Bodipy 581/591 C11 (Cell Signaling, 95978) was added directly to each well and incubated for 30 min
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