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Protein Assay CBB Solution (5 ) (Nacalai Tesque, Kyoto, Japan) was diluted fivefold with ion-exchanged water and dispensed in 1.5 mL portions into 2 mL tubes

Many describe it as the first time in their lives that they felt genuinely neutral about food able to leave half a meal without effort, uninterested in snacking, and free from the mental chatter around eating that had defined their relationship with food for years

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The transient receptor potential vanilloid 1 (TRPV1) channel plays a crucial role in heat pain perception 19