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The reaction between RT and GSH is based on a Michael addition reaction that is inherently reversible, has an appropriate dissociation equilibrium constant K d in the mM range and rapid reaction kinetics, and provides ratiometric readouts, thus allowing GSH quantification independent of the probe concentration
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The overall redox potential of a cell is primarily determined by oxidizable/reducible chemical pairs, including GSH/GSSH, reduced thioredoxin/oxidized thioredoxin, NAD+/NADH, and NADP+/NADPH, as shown in Fig
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