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It is the clinically accurate one
Briefly, at the end of the experiment, 20 l of the MTT reagent (EMD Millipore Corp, USA, Cat# 475989) was added to the medium and incubated for 3 h
It is used to increase range of motion in the PIP joint affected by tightness or contracture
The one thing you absolutely dont want to do is get so discouraged that you stop trying to lose weight at all, and revert to your old eating and exercise habits
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