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SAMe is then utilized as a donor of its methyl group to methylate many substrates to fulfill diverse biological functions

After blocking with blocking solution (0.2% casein, 0.3% Triton X-100, and 3% goat serum diluted in PBS) for 2 h at room temperature, anti-C3 (1:100 dilution) antibody-stained sections were reacted overnight at 4 C

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Several mechanisms contributing to increased oxidative stress in the diabetic milieu have been proposed, including glucose auto-oxidation, weakened antioxidant defense systems, metabolic abnormalities, and mitochondrial damage [45,46,47]