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PLoS One 7 , e29763 (2012)
A single week of low energy doesn't show much, but three weeks in a row suggest something worth looking into
It is a treatment for deficiency, not a general energy supplement
Methods Construct modification and expression of full-length GLP-1R The optimized gene of full-length GLP-1R was cloned into the modified pFastbac1 vector at the BamHI/HindIII sites and the native signal sequence was replaced by haemagglutinin (HA) to enhance receptor expression (see primers in Supplementary Table 2), followed by a flag-tag, a 10 His-tag and a tobacco etch virus protease (TEV) site at the N-terminus
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