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PubMed Research indicates that retinol has been employed in reconstructed human epidermis models to induce photo-oxidative stress, demonstrating its relevance in studying skin aging mechanisms

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To make the mutated binding site construct, four bases of the putative miR18a binding site on Gclc 3 UTR were mutated using sitedirected mutagenesis, as per manufacturer's instructions (QuikChange Lightning SiteDirected Mutagenesis Kit, Agilent)